| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Butyricicoccus pullicaecorum | TCTGGTCTGTGAAGCGCTG | TGTGCGCAGAAAAGCAGC | 60.01 | 59.67 | 151 | 176 |
100.00%
|
100.00%
|
| Primer 2 | Butyricicoccus pullicaecorum | GCGTTACTTGTCTGCCGTC | CGCACAAAACCATCCGTCC | 59.22 | 59.79 | 288 | 158 |
100.00%
|
100.00%
|
| Primer 3 | Butyricicoccus pullicaecorum | TATGTCCATGCGGTGCTGG | AGGCAAAGCGCAGGTCAT | 60.15 | 59.97 | 184 | 149 |
100.00%
|
100.00%
|
| Primer 4 | Butyricicoccus pullicaecorum | AAGGATCGCCTGCGCAT | AGGCAAAGCGCAGGTCAT | 59.43 | 59.97 | 211 | 149 |
100.00%
|
100.00%
|
| Primer 5 | Butyricicoccus pullicaecorum | AACCGATGGCATGCGTGT | GGTTGCATCGTCGCGAATC | 60.36 | 60.01 | 256 | 148 |
100.00%
|
100.00%
|
| Primer 6 | Butyricicoccus pullicaecorum | AGGTTGCTGCGGATGAGT | AAATGCAGCAACGTCGGC | 58.93 | 59.74 | 281 | 143 |
100.00%
|
100.00%
|
| Primer 7 | Butyricicoccus pullicaecorum | GCACATCGCCATCATCTGC | AAATGCAGCAACGTCGGC | 59.72 | 59.74 | 160 | 143 |
100.00%
|
100.00%
|
| Primer 8 | Butyricicoccus pullicaecorum | ACGACCGAACACCGTATGG | TTGCCATCTCAGCGCAGT | 59.79 | 59.65 | 298 | 143 |
100.00%
|
100.00%
|
| Primer 9 | Butyricicoccus pullicaecorum | ACGGCAGAATCGAGACCAC | TTGCCATCTCAGCGCAGT | 59.78 | 59.65 | 243 | 143 |
100.00%
|
100.00%
|
| Primer 10 | Butyricicoccus pullicaecorum | ATCGCCTGCGCATCGAT | AGGCAAAGCGCAGGTCAT | 59.60 | 59.97 | 207 | 141 |
100.00%
|
100.00%
|